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A fluorescent probe for detecting bisulfite/sulfite in lipid droplets and tracking the dynamics of lipid droplets.
Huang, Yan; Xin, Haotian; Lin, Qiaowen; Yang, Guiyi; Zhang, Yan; Cao, Duxia; Yu, Xiaoqiang.
Affiliation
  • Huang Y; School of Materials Science and Engineering, University of Jinan, Jinan, 250022, Shandong, China.
  • Xin H; School of Materials Science and Engineering, University of Jinan, Jinan, 250022, Shandong, China.
  • Lin Q; State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, Shandong, China.
  • Yang G; School of Materials Science and Engineering, University of Jinan, Jinan, 250022, Shandong, China.
  • Zhang Y; School of Materials Science and Engineering, University of Jinan, Jinan, 250022, Shandong, China.
  • Cao D; School of Materials Science and Engineering, University of Jinan, Jinan, 250022, Shandong, China. Electronic address: duxiacao@ujn.edu.cn.
  • Yu X; State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, Shandong, China. Electronic address: yuxq@sdu.edu.cn.
Talanta ; 279: 126605, 2024 Nov 01.
Article in En | MEDLINE | ID: mdl-39084038
ABSTRACT
Intracellular lipid droplets (LDs) are important organelles regulating intracellular redox processes. Endogenous bisulfite/sulfite (HSO3-/SO32-) is one of the metabolites of thiol metabolism. The variation in HSO3-/SO32- content around LDs is closely related to cellular homeostasis. However, there is currently no effective method to visualize and quantify the dynamic changes in HSO3-/SO32- content around LDs. In this work, a fluorescent probe MC-BEN utilizing a triphenylamine basic framework was developed to selectively recognize HSO3-/SO32- via a nucleophilic addition reaction. The probe exhibits excellent anti-interference capability, short response time, outstanding photostability, and a low fluorescence detection limit (6.1 µM) for HSO3-/SO32- recognition. More interesting, there is a trend of accelerated contact between LDs and lysosomes after MC-BEN targeting LDs and reacting with endogenous/exogenous HSO3-/SO32-, which may provide new ideas for the study of intracellular lysosomal lipophagy.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sulfites / Lipid Droplets / Fluorescent Dyes Limits: Humans Language: En Journal: Talanta Year: 2024 Document type: Article Affiliation country: Country of publication:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sulfites / Lipid Droplets / Fluorescent Dyes Limits: Humans Language: En Journal: Talanta Year: 2024 Document type: Article Affiliation country: Country of publication: