Atrophy-Independent and Dependent Iron and Myelin Changes in Deep Gray Matter of Multiple Sclerosis: A Longitudinal Study Using χ-Separation Imaging.

ABSTRACT

RATIONALE AND OBJECTIVES: To investigate iron and myelin changes in deep gray matter (DGM) of relapsing-remitting multiple sclerosis (RRMS) patients and their relationship to atrophy by χ-separation imaging. MATERIALS AND METHODS: 33 RRMS patients and 34 healthy controls (HC) were included in this study. The χ-separation map reconstructed from a 3D multi-echo gradient echo scan was used to measure the positive susceptibility (χpos) and negative susceptibility (χneg) of DGM. To take into account the effect of atrophy, susceptibility mass of DGM was calculated by multiplying volume by the mean bulk susceptibility. Differences in MRI metrics between baseline patients, follow-up patients, and HC were compared respectively. RESULTS: Compared to HC, χpos of basal ganglia were significantly increased in follow-up patients (P < 0.05). The χpos of pallidum was significantly higher in follow-up patients than that in baseline patients (P = 0.006). The χneg of caudate, pallidum and hippocampus in baseline and follow-up patients was significantly higher than that in HC (P < 0.05). When taking into account the effect of atrophy, there was a significant decrease in χpos mass and a significant increase in χneg mass of thalamus, accumbens and amygdala in follow-up patients compared to HC (P < 0.05). The χpos mass of the thalamus was further decreased in follow-up patients compared to baseline patients (P = 0.006). CONCLUSION: χ-separation imaging could generate independent information on iron and myelin changes in RRMS patients, showing atrophy-dependent iron increase in basal ganglia and atrophy-independent iron and myelin decrease in thalamus.