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Exploiting CO2 laser to boost graphite inks electron transfer for fructose biosensing in biological fluids.
Silveri, Filippo; Della Pelle, Flavio; Scroccarello, Annalisa; Bollella, Paolo; Ferraro, Giovanni; Fukawa, Eole; Suzuki, Yohei; Sowa, Keisei; Torsi, Luisa; Compagnone, Dario.
Affiliation
  • Silveri F; Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Campus "Aurelio Saliceti" Via R. Balzarini 1, 64100, Teramo, Italy.
  • Della Pelle F; Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Campus "Aurelio Saliceti" Via R. Balzarini 1, 64100, Teramo, Italy. Electronic address: fdellapelle@unite.it.
  • Scroccarello A; Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Campus "Aurelio Saliceti" Via R. Balzarini 1, 64100, Teramo, Italy.
  • Bollella P; Department of Chemistry, University of Bari Aldo Moro, Via E. Orabona 4, 70125, Bari, Italy.
  • Ferraro G; Department of Chemistry "Ugo Schiff" and CSGI, University of Florence, Via Della Lastruccia 3, Sesto Fiorentino, 50019, Florence, Italy.
  • Fukawa E; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Suzuki Y; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Sowa K; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan.
  • Torsi L; Department of Chemistry, University of Bari Aldo Moro, Via E. Orabona 4, 70125, Bari, Italy.
  • Compagnone D; Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Campus "Aurelio Saliceti" Via R. Balzarini 1, 64100, Teramo, Italy. Electronic address: dcompagnone@unite.it.
Biosens Bioelectron ; 263: 116620, 2024 Nov 01.
Article in En | MEDLINE | ID: mdl-39094288
ABSTRACT
The possibility to print electronics by means of office tools has remarkedly increased the possibility to design affordable and robust point-of-care/need devices. However, conductive inks suffer from low electrochemical and rheological performances limiting their applicability in biosensors. Herein, a fast CO2 laser approach to activate printed carbon inks towards direct enzymatic bioelectrocatalysis (3rd generation) is proposed and exploited to build biosensors for D-fructose analysis in biological fluids. The CO2 laser treatment was compared with two lab-grade printed transducers fabricated with solvent (SB) and water (WB) based carbon inks. The use of the laser revealed significant morpho-chemical variations on the printed inks and was investigated towards enzymatic direct catalysis, using Fructose dehydrogenase (FDH) integrated into entirely lab-produced biosensors. The laser-driven activation of the inks unveils the inks' direct electron transfer (DET) ability between FDH and the electrode surface. Sub-micromolar limits of detection (SB-ink LOD = 0.47 µM; WB-ink LOD = 0.24 µM) and good linear ranges (SB-ink 5-100 µM; WB-ink 1-50 µM) were obtained, together with high selectivity due to use of the enzyme and the low applied overpotential (0.15 V vs. pseudo-Ag/AgCl). The laser-activated biosensors were successfully used for D-fructose determination in complex synthetic and real biological fluids (recoveries 93-112%; RSD ≤8.0%, n = 3); in addition, the biosensor ability for continuous measurement (1.5h) was also demonstrated simulating physiological D-fructose fluctuations in cerebrospinal fluid.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biosensing Techniques / Fructose / Graphite / Ink Limits: Humans Language: En Journal: Biosens Bioelectron Journal subject: BIOTECNOLOGIA Year: 2024 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biosensing Techniques / Fructose / Graphite / Ink Limits: Humans Language: En Journal: Biosens Bioelectron Journal subject: BIOTECNOLOGIA Year: 2024 Document type: Article Affiliation country: Country of publication: