Development of Hypoallergenic Derivatives of Cra a 1 with B Cell Epitope Deletion and T Cell Epitope Retention.
J Agric Food Chem
; 72(35): 19494-19504, 2024 Sep 04.
Article
in En
| MEDLINE
| ID: mdl-39168117
ABSTRACT
Tropomyosin was reported as an important allergen in Crassostrea angulata and designated as Cra a 1. The localization of the T cell epitopes and the reduction of the immunoreactivity of Cra a 1 are still lacking. In this study, four T cell epitopes were identified by using wild-type Cra a 1 (wtCra a 1)-immunized mouse splenocytes cultured with synthetic peptides. The immunoreactivity was maintained after chemical denaturation treatment, indicating that the linear epitope is an immunodominant epitope of wtCra a 1. Furthermore, the hypoallergenic derivative (mCra a 1) was developed by the deletion of linear B cell epitopes and retention of T cell epitopes. mCra a 1 could stimulate CD4+T cell proliferation and upregulate interleukin-10 secretion. Overall, basophil activation by mCra a 1 was low, but its ability to induce T cell proliferation was retained, suggesting that mCra a 1 may serve as a viable candidate for treating oyster allergy.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Allergens
/
Epitopes, T-Lymphocyte
/
Epitopes, B-Lymphocyte
/
Crassostrea
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
J Agric Food Chem
/
J. agric. Food chem
/
Journal of agricultural and food chemistry
Year:
2024
Document type:
Article
Affiliation country:
Country of publication: