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VitelloTag: a tool for high-throughput cargo delivery into oocytes.
Clarke, D Nathaniel; Kane, Akshay; Perillo, Margherita; Lowe, Christopher J; Swartz, S Zachary.
Affiliation
  • Clarke DN; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142,USA.
  • Kane A; The Eugene Bell Center for Regenerative Biology and Tissue Engineering, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543-1015, USA.
  • Perillo M; The Eugene Bell Center for Regenerative Biology and Tissue Engineering, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543-1015, USA.
  • Lowe CJ; Hopkins Marine Station, Stanford University, Pacific Grove, CA 93950,USA.
  • Swartz SZ; The Eugene Bell Center for Regenerative Biology and Tissue Engineering, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543-1015, USA.
Development ; 151(20)2024 Oct 15.
Article in En | MEDLINE | ID: mdl-39171380
ABSTRACT
Delivering molecular tools into oocytes is essential for developmental and reproductive biology. Microinjection, the conventional method, is equipment intensive, often technically challenging and has a low yield, and is impractical in species with delicate oocytes or restricted spawning seasons. To overcome these limitations, we developed VitelloTag, a cost-effective, high-throughput system using vitellogenin-derived fusion proteins to enable efficient cargo delivery via receptor-mediated endocytosis. We demonstrate its utility by delivering Cas9/sgRNA complexes in two distantly related species for gene knockout.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oocytes / Vitellogenins Limits: Animals Language: En Journal: Development / Development (Camb.) / Development (Cambridge) Journal subject: BIOLOGIA / EMBRIOLOGIA Year: 2024 Document type: Article Affiliation country: Country of publication:
Fulltext
Add to My VHL
Print
XML
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oocytes / Vitellogenins Limits: Animals Language: En Journal: Development / Development (Camb.) / Development (Cambridge) Journal subject: BIOLOGIA / EMBRIOLOGIA Year: 2024 Document type: Article Affiliation country: Country of publication: