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Spatiotemporal dynamics of microscopic biological barrier visualized by electric-double-layer modulation imaging.
Kurosu, Jun; Sakamaki, Takato; Kanai, Kaname; Morishita, Kana; Sumaru, Kimio; Tsutsumi, Jun'ya.
Affiliation
  • Kurosu J; Research Institute for Applied Electronics and Photonics, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, 305-8565, Japan; Department of Physics and Astronomy, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, 278-85
  • Sakamaki T; Research Institute for Applied Electronics and Photonics, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, 305-8565, Japan; Department of Physics and Astronomy, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, 278-85
  • Kanai K; Department of Physics and Astronomy, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, 278-8510, Japan.
  • Morishita K; Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, 305-8565, Japan.
  • Sumaru K; Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, 305-8565, Japan.
  • Tsutsumi J; Research Institute for Applied Electronics and Photonics, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, 305-8565, Japan; Department of Physics and Astronomy, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, 278-85
Biosens Bioelectron ; 266: 116721, 2024 Dec 15.
Article in En | MEDLINE | ID: mdl-39226753
ABSTRACT
Live-cell label-free imaging of a microscopic biological barrier, generally referred to as 'tight junction', was realized by a recently developed electric-double-layer modulation imaging (EDLMI). The method allowed quantitative imaging of barrier integrity in real time, thus being an upper compatible of transepithelial electrical resistance (TEER) which is a conventional standard technique to evaluate spatially averaged barrier integrity. We demonstrate that the quantitative and real-time imaging capability of EDLMI unveils fundamental dynamics of biological barrier, some of which are totally different from conventional understandings.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biosensing Techniques Limits: Humans Language: En Journal: Biosens Bioelectron Journal subject: BIOTECNOLOGIA Year: 2024 Document type: Article Country of publication:
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biosensing Techniques Limits: Humans Language: En Journal: Biosens Bioelectron Journal subject: BIOTECNOLOGIA Year: 2024 Document type: Article Country of publication: