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Cellular and In Vivo Response to Industrial, Food Grade, and Photocatalytic TiO2 Nanoparticles.
Heckman, Morgan M; Albright, Michaela C; Poulsen, Karsten M; Tighe, Robert M; Payne, Christine K.
Affiliation
  • Heckman MM; Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina 27708, United States.
  • Albright MC; Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, United States.
  • Poulsen KM; Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina 27708, United States.
  • Tighe RM; Department of Medicine, Duke University School of Medicine, Durham, North Carolina 27710, United States.
  • Payne CK; Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina 27708, United States.
J Phys Chem B ; 128(37): 8878-8885, 2024 Sep 19.
Article in En | MEDLINE | ID: mdl-39231525
ABSTRACT
We encounter titanium dioxide nanoparticles (TiO2 NPs) throughout our daily lives in the form of food coloring, cosmetics, and industrial materials. They are used on a massive industrial scale, with over 1 million metric tons in the global market. For the workers who process these materials, inhalation is a major concern. The goal of our current research is to provide a direct comparison of the three major types of TiO2 NPs (P25, E171, R101) in terms of surface characterization, cellular response, and in vivo response following introduction into the lungs of mice. In both cellular and in vivo experiments, we observe a pro-inflammatory response to the P25 TiO2 NPs that is not observed in the E171 or R101 TiO2 NPs at mass-matched concentrations. Cellular experiments measured a cytokine, TNF-α, as a marker of a pro-inflammatory response. In vivo experiments in mice measured the number of immune cells and four pro-inflammatory cytokines (IL-6, MIP-2, IP-10, and MCP-1) present in bronchoalveolar lavage fluid. A detailed physical and chemical characterization of the TiO2 NPs shows that the P25 TiO2 NPs are distinguished by smaller primary particles suggesting that samples matched by mass contain a larger number of P25 TiO2 NPs. Cellular dose-response measurements with the P25, E171, and R101 TiO2 NPs support this hypothesis showing increased TNF-α release by macrophages as a function of TiO2 NP dose. Overall, this direct comparison of the three major types of TiO2 NPs shows that the number of particles in a dose, which is dependent on the particle diameter, is a key parameter in TiO2 NP-induced inflammation.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Titanium Limits: Animals Language: En Journal: J Phys Chem B Journal subject: QUIMICA Year: 2024 Document type: Article Affiliation country: Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Titanium Limits: Animals Language: En Journal: J Phys Chem B Journal subject: QUIMICA Year: 2024 Document type: Article Affiliation country: Country of publication: