Non-viral approaches in CAR-NK cell engineering: connecting natural killer cell biology and gene delivery.
J Nanobiotechnology
; 22(1): 552, 2024 Sep 10.
Article
in En
| MEDLINE
| ID: mdl-39256765
ABSTRACT
Natural Killer (NK) cells are exciting candidates for cancer immunotherapy with potent innate cytotoxicity and distinct advantages over T cells for Chimeric Antigen Receptor (CAR) therapy. Concerns regarding the safety, cost, and scalability of viral vectors has ignited research into non-viral alternatives for gene delivery. This review comprehensively analyses recent advancements and challenges with non-viral genetic modification of NK cells for allogeneic CAR-NK therapies. Non-viral alternatives including electroporation and multifunctional nanoparticles are interrogated with respect to CAR expression and translational responses. Crucially, the link between NK cell biology and design of drug delivery technologies are made, which is essential for development of future non-viral approaches. This review provides valuable insights into the current state of non-viral CAR-NK cell engineering, aimed at realising the full potential of NK cell-based immunotherapies.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Killer Cells, Natural
/
Immunotherapy, Adoptive
/
Gene Transfer Techniques
/
Cell Engineering
/
Receptors, Chimeric Antigen
Limits:
Animals
/
Humans
Language:
En
Journal:
J Nanobiotechnology
Year:
2024
Document type:
Article
Country of publication: