Supported liquid phase extraction using wetted nanofiber discs as a simple approach for in-vial extraction of chlorophenols and insecticides from river water prior to HPLC-DAD analysis.
Anal Chim Acta
; 1327: 343137, 2024 Oct 23.
Article
in En
| MEDLINE
| ID: mdl-39266060
ABSTRACT
BACKGROUND:
A novel supported liquid extraction approach using small polymeric nanofibrous discs was demonstrated and applied to the analysis of real river water. Nanofibrous discs were tested to extract model mixture of 9 common water contaminants 4-nitrophenol, various chlorophenols, bisphenol A, permethrin, and fenoxycarb featuring a wide range of log P values (1.9-6.5). Polyacrylonitrile, polyhydroxybutyrate, and polylactic acid nanofibers were selected as adsorptive materials. One-step desorption was performed directly in HPLC vials, to avoid time-consuming evaporation and reconstitution steps. The discs were allowed to sediment to the bottom of the vial before injection into the chromatographic system.RESULTS:
Various parameters affecting the extraction efficiency including 1-octanol volume, extraction time, ionic strength, and sample volume were investigated and optimized. Wetting the nanofiber discs with 1-octanol resulted in up to 20-fold increase in enrichment factor when compared to non-wetted polymer counterparts. The highest enrichment factors were observed for analytes with a log P range of 3.3-4.5. Our developed method showed good linearity in the range 20-200 µg/L for all analytes tested. Satisfactory repeatability with RSD <13 % were achieved covering all steps including disc preparation, wetting, extraction/elution, and chromatography analysis, and recoveries ranged from 58.93 to 121.43 %.SIGNIFICANCE:
This work represents novel simple supported liquid extraction approach using impregnated polymer nanofiber discs. Using only 50 µL 1-octanol, we reduced the organic solvent compared to other extraction methods. There was no need for any plastic cartridge to hold the sorbent and direct in-vial desorption reduced the unnecessary, time-consuming steps and simplified the sample preparation protocol.
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Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Anal Chim Acta
Year:
2024
Document type:
Article
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