Determination of tamoxifen and metabolites in human serum by high-performance liquid chromatography with post-column fluorescence activation.
J Chromatogr
; 272(2): 351-8, 1983 Feb 11.
Article
in En
| MEDLINE
| ID: mdl-6833432
ABSTRACT
Sensitive and reproducible analyses were developed for assaying tamoxifen, monohydroxytamoxifen, N-desmethyltamoxifen, metabolite E [trans-1(4-hydroxyphenyl)1,2-diphenylbut-1-ene] and a new metabolite, metabolite Y [trans-1(4-hydroxyethoxyphenyl)-1,2-diphenylbut-1-ene] in human serum using high-performance liquid chromatography (HPLC). Three different systems were developed for specific purposes. All chromatography was performed using serum extracts made with hexane-butanol. Detection was by fluorimetry of phenanthrene derivatives formed by on-stream UV irradiation with a newly described device for post-column irradiation of the HPLC stream. This device may be of use in other HPLC systems requiring post-column photochemical reactions.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tamoxifen
Limits:
Animals
/
Humans
Language:
En
Journal:
J Chromatogr
Year:
1983
Document type:
Article