Murine hybridoma-derived antibodies in the processing of antigens for the immunodiagnosis of hydatid (Echinococcus granulosus) infection in sheep.
Parasitology
; 83(Pt 2): 303-17, 1981 Oct.
Article
in En
| MEDLINE
| ID: mdl-7329721
ABSTRACT
Mice experimentally infected with hydatid parasite Echinococcus granulosus (secondary echinococcosis), were used as spleen cell donors for hybridoma production. Two hybridomas were produced which secreted antibody with anti-E. granulosus cyst fluid (EgCF) activity. Radioimmunoassays comparing the binding of these hybridoma-derived antibodies to E. granulosus versus several other antigen preparations from sheep parasites showed that the antibodies had a high degree of specificity for the hydatid parasite. However, using a panel of clinically defined sheep sera in a competitive radio-immunoassay, the binding of the hybridoma-derived antibodies to EgCF was inhibited strongly by sera from sheep infected with the common parasites Fasciola hepatica and Taenia hydatigena. When the hybridoma-derived antibodies were conjugated to CNBr-activated Sepharose and the crude EgCF applied to the affinity column, the "run through' antigen preparation showed a significant increase in specificity for experimental E. granulosus infection. A useful means of applying the hybridoma technology in the development of new immunodiagnostic reagents may be to select those murine hybridomas which produce high-affinity, cross-reacting antibodies and to use these antibodies in the processing of homologous antigen by affinity chromatography.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sheep Diseases
/
Echinococcosis
/
Antibodies
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Parasitology
Year:
1981
Document type:
Article