Binding studies with 3H-atropine in intact isolated guinea pig gastric mucosal cells.

ABSTRACT

3H-atropine binding was studied in isolated intact guinea pig gastric mucosal cells and has been shown to be saturable. Scatchard plots of the binding system calculated under the assumption of only one binding site revealed KD values for the parietal cell enriched population of 1.25 x 10(-6) mol/l and 1.38 x 10(-6) mol/l for the nonparietal cells. The results show that in gastric mucosa both parietal and nonparietal cells contain cholinergic receptors.