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Quantitative polymerase chain reaction assay for aggrecan and link protein gene expression in cartilage.
Re, P; Valhmu, W B; Vostrejs, M; Howell, D S; Fischer, S G; Ratcliffe, A.
Affiliation
  • Re P; Department of Orthopaedic Surgery, Columbia University, New York, New York 10027, USA.
Anal Biochem ; 225(2): 356-60, 1995 Mar 01.
Article in En | MEDLINE | ID: mdl-7762805
ABSTRACT
A procedure has been developed to quantify the levels of aggrecan and link protein mRNAs in small amounts of various tissues, including cartilage, using the power of PCR to amplify extremely low levels of specific templates. The PCR protocol which was selected allows for a simple assay procedure, with standards in different tubes from the samples. This straightforward procedure is quantitative, inexpensive, and allows for many samples to be analyzed at one time.
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Collection: 01-internacional Database: MEDLINE Main subject: Proteoglycans / Proteins / Cartilage / Gene Expression Regulation / Polymerase Chain Reaction / Extracellular Matrix Proteins Limits: Animals Language: En Journal: Anal Biochem Year: 1995 Document type: Article Affiliation country:
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PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: Proteoglycans / Proteins / Cartilage / Gene Expression Regulation / Polymerase Chain Reaction / Extracellular Matrix Proteins Limits: Animals Language: En Journal: Anal Biochem Year: 1995 Document type: Article Affiliation country: