Identification of disulfide bridges in a cardiotoxic peptide by electrospray ionization.
Biol Mass Spectrom
; 23(12): 719-26, 1994 Dec.
Article
in En
| MEDLINE
| ID: mdl-7841206
ABSTRACT
A new method was developed for subjecting a peptide to a specified number of Edman degradation cycles on an automated polypeptide sequencer and desorbing the residual peptide for further investigations. The procedure was applied in combination with electrospray ionization mass spectrometry to identify the four disulfide bridges present in a small tightly bound peptide. The task was accomplished using only a few nanomoles of the intact peptide.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Mass Spectrometry
/
Disulfides
Type of study:
Diagnostic_studies
Language:
En
Journal:
Biol Mass Spectrom
Journal subject:
BIOQUIMICA
Year:
1994
Document type:
Article