Sulofenur cytotoxicity and changes in cytosolic calcium and mitochondrial membrane potential in human colon adenocarcinoma cell lines.
Cancer Lett
; 88(1): 27-35, 1995 Jan 06.
Article
in En
| MEDLINE
| ID: mdl-7850770
ABSTRACT
Sulofenur treatment (12.5 microM-1 mM) of colon adenocarcinoma cell lines resulted in dose- and time-dependent cell killing. LYc5 cells were viable longer than GC3/c1 cells. Each concentration resulted in elevation of cytosolic calcium [Ca2+]i) for both cell lines. At lower doses, elevation was delayed for LYc5 cells. GC3/c1 cells after 1 mM treatment in Ca(2+)-free HBSS showed no rise of [Ca2+]i. GC3/c1 cells after carbonyl cyanide-m-chlorophenylhydrazone rapidly lost rhodamine 123 fluorescence from mitochondria; after 1 mM sulofenur, fluorescence faded slowly. Following treatment, cells became rounded, blebs formed and the cells died. Results suggest that elevated [Ca2+]i plays an important role in sulofenur cytotoxicity.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sulfonylurea Compounds
/
Adenocarcinoma
/
Calcium
/
Colonic Neoplasms
/
Mitochondria
Limits:
Humans
Language:
En
Journal:
Cancer Lett
Year:
1995
Document type:
Article