H-ras transfection of the rat kidney cell line NRK-52E results in increased induction of c-fos, c-jun and hsp70 following sulofenur treatment.
Cancer Lett
; 106(2): 199-205, 1996 Sep 10.
Article
in En
| MEDLINE
| ID: mdl-8844973
ABSTRACT
The effect of the antineoplastic drug sulofenur on the induction of the immediate-early genes (IEG) c-fos and c-jun and the stress gene hsp70 was compared in the rat kidney epithelial-like cell line NRK-52E and a derivative H-ras-transfected (H/1.2NRK-52E) cell line. Fold induction for each gene after sulofenur (500 microM) treatment was greater in H/1.2NRK-52E. The maximum increases for NRK-2E and H/1.2NRK-52E were as follows c-fos, approximately 10-fold and approximately 18-fold; c-jun, approximately 2.5-fold and approximately 3.6-fold; hsp70, approximately 13-fold and approximately 30-fold. In cells loaded with EGTA/AM or treated in low or no Ca2+ HBSS, c-fos induction was reduced similarly in both cell types. However, inhibition of protein kinases with staurosporin and calphostin C reduced c-fos by 80% in NRK-52E but by only 10-20% in H/1.2NRK.52E. These results indicate that sulofenur-induced IEG elevation is Ca(2+)-dependent and that the requirement for protein kinase C activation is bypassed in H-ras-transfected cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sulfonylurea Compounds
/
Gene Expression Regulation
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Genes, ras
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Genes, jun
/
Genes, fos
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HSP70 Heat-Shock Proteins
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Antineoplastic Agents
Limits:
Animals
Language:
En
Journal:
Cancer Lett
Year:
1996
Document type:
Article
Affiliation country: