High-performance liquid chromatographic determination of midazolam in rat brain.
J Chromatogr B Biomed Appl
; 683(2): 276-80, 1996 Aug 30.
Article
in En
| MEDLINE
| ID: mdl-8891927
ABSTRACT
A high-performance liquid chromatography method for the determination of midazolam in rat brain is described. Midazolam and the internal standard halazepam were extracted with toluene and analyzed isocratically on a reversed-phase column with a mobile phase consisting of methanol, acetonitrile and potassium phosphate buffer. Detection was monitored by ultraviolet absorption at 240 nm. The standard curves were linear over the range of 25-350 ng midazolam per 50 mg brain tissue. The day-to-day coefficient of variation ranged from 1.7 to 6.9%. The limit of quantification was 80 ng/g brain tissue. The method is rapid, simple and reproducible for brain analysis.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Anti-Anxiety Agents
/
Midazolam
/
Brain Chemistry
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Chromatogr B Biomed Appl
Year:
1996
Document type:
Article
Affiliation country: