Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol
; 178(21): 6361-5, 1996 Nov.
Article
in En
| MEDLINE
| ID: mdl-8892842
ABSTRACT
Northern (RNA) blot analysis of the Bacillus subtilis biotin operon, bioWAFDBIorf2, detected at least two steady-state polycistronic transcripts initiated from a putative vegetative (Pbio) promoter that precedes the operon, i.e., a full-length 7.2-kb transcript covering the entire operon and a more abundant 5.1-kb transcript covering just the first five genes of the operon. Biotin and the B. subtilis birA gene product regulated synthesis of the transcripts. Moreover, replacing the putative Pbio promoter and regulatory sequence with a constitutive SP01 phage promoter resulted in higher-level constitutive synthesis. Removal of a rho-independent terminator-like sequence located between the fifth (bioB) and sixth (bioI) genes prevented accumulation of the 5.1-kb transcript, suggesting that the putative terminator functions to limit expression of bioI, which is thought to be involved in an early step in biotin synthesis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Operon
/
Bacillus subtilis
/
Biotin
/
RNA, Bacterial
Type of study:
Diagnostic_studies
Language:
En
Journal:
J Bacteriol
Year:
1996
Document type:
Article
Affiliation country: