Nonsuperoxide lucigenin-enhanced chemiluminescence from phospholipids and human saphenous veins.
Free Radic Biol Med
; 18(3): 565-9, 1995 Mar.
Article
in En
| MEDLINE
| ID: mdl-9101248
ABSTRACT
The use of lucigenin-enhanced chemiluminescence (CL) for the detection of superoxide (O2.-) has grown in popularity due to an increased demand for a simple and specific system capable of measuring superoxide. In this study we report a lucigenin-CL signal emanating from human saphenous veins (SV) that was not inhibited by superoxide dismutase (SOD) and lasted for more than 24 h. A larger CL-signal with similar properties was produced by saphenous veins that had been dehydrated. A similar, non-SOD-inhibitable lucigenin-CL was also produced with a variety of phospholipids and phosphatidic acid. The chemical moiety responsible for the phospholipid CL is oxygen dependent but remains unidentified because a variety of lipids and phosphate containing species failed to produce such a signal. These results suggest that the use of lucigenin as a specific CL enhancer for O2.- must be clearly discriminated with a specific O2.- inhibitor when used in biological systems.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phospholipids
/
Saphenous Vein
/
Acridines
/
Superoxides
Limits:
Humans
Language:
En
Journal:
Free Radic Biol Med
Journal subject:
BIOQUIMICA
/
MEDICINA
Year:
1995
Document type:
Article
Affiliation country: