Immunocytochemical identification of spore proteins in two microsporidia, with emphasis on extrusion apparatus.
J Eukaryot Microbiol
; 45(2): 224-31, 1998.
Article
in En
| MEDLINE
| ID: mdl-9561775
ABSTRACT
Microsporidia can form small spores with a unique invasive apparatus featuring a long polar tube whose extrusion allows entry of infectious sporoplasm into a host cell. The reactivity of mouse polyclonal antibodies raised against sporal proteins from two microsporidian species belonging to different genera (Glugea atherinae and Encephalitozoon cuniculi) was studied by western blotting and indirect immunofluorescence. Whole protein antisera provided a few cross-reactions relatable to some proteins of the spore envelope or polar tube. Ultrastructural immunocytochemistry with murine antibodies against protein bands separated by sodium dodecylsulphate polyacrylamide gel electrophoresis allowed the assignment of several proteins to the polar tube (34, 75 and 170 kDa in Glugea, 35, 55 and 150 kDa in Encephalitozoon). Antigenic similarities were detected for the Glugea 34 kDa and Encephalitozoon 35 kDa polar tube proteins. Species-specific proteins were shown to be located in either the lamellar polaroplast of Glugea or the spore envelope of Encephalitozoon.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protozoan Proteins
/
Microsporida
/
Encephalitozoon cuniculi
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
J Eukaryot Microbiol
Journal subject:
MICROBIOLOGIA
/
PARASITOLOGIA
Year:
1998
Document type:
Article
Affiliation country: