HPLC-fluorescence determination of unconjugated estrogens in pharmaceuticals.
J Pharm Biomed Anal
; 18(1-2): 187-92, 1998 Oct.
Article
in En
| MEDLINE
| ID: mdl-9863957
ABSTRACT
A fluorimetric liquid chromatographic method (lambda(ex) = 280 nm; lambda(em) = 312 nm) was developed for measurements of unconjugated estrogens (estradiol and estriol) in pharmaceutical dosage forms using a reversed-phase column with water acetonitrile at different composition as mobile phase. The in vitro release profiles of three different estradiol transdermal therapeutic systems were determined through a medical-grade silicone rubber subdermal implant material membrane, using a modified Franz diffusion apparatus at 37 degrees C in presence of PEG 400. The HPLC method possesses advantages of rapidity, simplicity and accuracy.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chemistry, Pharmaceutical
/
Estradiol
/
Estriol
Language:
En
Journal:
J Pharm Biomed Anal
Year:
1998
Document type:
Article
Affiliation country: