Cytokine production during the inhibition of acute vascular rejection in a concordant hamster-to-rat cardiac xenotransplantation model / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 145-149, 2007.
Article
in En
| WPRIM
| ID: wpr-273320
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>The aim of the current study was to investigate the role of interleukin (IL)-2, interferon (IFN)-gamma, IL-4 and IL-10 in a concordant hamster-to-rat cardiac xenotransplantation model.</p><p><b>METHODS</b>A hamster-to-rat cardiac transplantation was performed using SD rats as recipients of Golden Syrian hamster hearts. A total of 60 SD rats were divided into four groups and treated as follows: control group (n = 15); splenectomy group (n = 15); CsA group (n = 15); CsA + splenectomy group (n = 15). Levels of IL-2, IFN-gamma, IL-4 and IL-10 were measured by enzyme linked immunosorbent assay (ELISA). Sera were harvested at different time points in each group: day 1, and 3 as well as the day the xenograft stopped beating in the control group and CsA group; day 1, 3, 7, 14 and 30 in the splenectomy group and CsA + splenectomy group. The expression of P-selectin and intercellular adhesion molecule-1 (ICAM-1) was examined by immunohistochemical analysis of the xenograft after cardiac xenotransplantation.</p><p><b>RESULTS</b>Serum levels of IL-2 and IFN-gamma were upregulated in untreated (day 3) and splenectomy-treated animals (day 7) compared to CsA + splenectomy treated animals (day 7). IL-10 was upregulated in long-term survival recipients following splenectomy + CsA. Neither P-selectin nor ICAM-1 expression was detected in long-term survival xenografts.</p><p><b>CONCLUSIONS</b>Serum IL-2 and IFN-gamma were elevated following acute vascular rejection. Serum IL-10 was correlated to immunosuppression and protective effects in long-term survival rats following concordant cardiac xenotransplantation.</p>
Full text:
1
Database:
WPRIM
Main subject:
Transplantation, Heterologous
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Immunohistochemistry
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Acute Disease
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Cytokines
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Heart Transplantation
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Mesocricetus
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Allergy and Immunology
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Graft Rejection
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Graft Survival
Limits:
Animals
Language:
En
Journal:
Chinese Medical Journal
Year:
2007
Document type:
Article