Preparation and identification of recombinant maxadilan / 生物工程学报
Chinese Journal of Biotechnology
; (12): 2049-2055, 2008.
Article
in Zh
| WPRIM
| ID: wpr-302874
Responsible library:
WPRO
ABSTRACT
To produce recombinant Maxadilan using gene engineering technology, the gene of recombinant Maxadilan which expressed in protocaryon were designed and synthesized according to the amino acid sequences of Maxadilan. The recombinant plasmid pKYB-MAX was constructed and transformed into host bacteria Escherichia coli strain ER2566. After the MAX-intein-CBD fusion protein was purified by chintin-affinity chromatography, the self-cleavage activity of the intein was induced by beta-mercaptoethanol and the recombinant Maxadilan was released from the chitin-bound intein tag. The molecular weight of peptides was determined by the laser flight mass spectrometry and the results was conformity with the theoretical value. The biological activity analysis showed that recombinant Maxadilan significantly enhanced the concentration of serum glucose.
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
/
Recombinant Proteins
/
Molecular Sequence Data
/
Base Sequence
/
Insect Proteins
/
Inteins
/
Escherichia coli
/
Genetics
/
Isopropyl Thiogalactoside
/
Metabolism
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2008
Document type:
Article