Experimental study on expression of GM-CSF from human endothelial cells and monocytes induced by total saponins of panax ginseng / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 845-847, 2003.
Article
in Zh
| WPRIM
| ID: wpr-320298
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of total saponins of Panax ginseng (TSPG) on the expression of granulocytemacrophage colony-stimulating factor (GM-CSF) from human endothelial cells and monocytes and the relationship between TSPG and human granulocytopoiesis and monocytopoiesis modulation.</p><p><b>METHODS</b>Adopting the hematopoietic progenitor cells culture in vitro, hematopoietic growth factor biological assay, immunocytochemistry and nucleic acid in situ hybridization, the GM-CSF expression in the endothelial cells and monocytes were detected.</p><p><b>RESULTS</b>The conditioned cultural media of endothelial and monocytes induced and prepared by TSPG, could significantly promote the proliferation and differentiation of human colony forming unit-granulocyte macrophage (CFU-GM), and enhance the protein and mRNA expression of GM-CSF in endothelial cells and monocytes.</p><p><b>CONCLUSION</b>TSPG could possibly through direct or indirect route, promote hematopoietic, induce endothelial cells and monocytes in the microenvironment to synthetize and secrete GM-CSF, so as to further promote the proliferation and differentiation of human CFU-GM.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
/
Saponins
/
Bone Marrow Cells
/
Monocytes
/
Cells, Cultured
/
Chemistry
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Culture Media, Conditioned
/
Cell Biology
/
Ginsenosides
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2003
Document type:
Article