Recombinant adeno-associated virus vector related impurities / 生物工程学报
Chinese Journal of Biotechnology
; (12): 717-723, 2011.
Article
in Zh
| WPRIM
| ID: wpr-324545
Responsible library:
WPRO
ABSTRACT
Recombinant adeno-associated virus (rAAV)-based vectors that can stably express therapeutic genes in vivo without detectable side-effect have shown great promise for human gene therapy. A major challenge for translation of promising research to clinical development is how to establish clinically compatible purification methods in separating rAAV from potentially pathogenic impurities, especially rAAV vector-related impurities, a class of impurities corresponding to AAV particles that closely resemble bona fide vectors and are difficult to remove. In this review we summarize the assembly process of rAAV vector-related impurities and their characteristics differed with rAAV vectors, and evaluate several current technologies to prevent their formation or separate them from rAAV stocks.
Full text:
1
Database:
WPRIM
Main subject:
Physiology
/
Recombination, Genetic
/
Virion
/
Virus Replication
/
Genetic Therapy
/
Dependovirus
/
Virus Assembly
/
Capsid Proteins
/
Genetic Vectors
/
Genetics
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2011
Document type:
Article