Epitope screening of influenza A (H3N2) by using phage display library / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 272-274, 2009.
Article
in Zh
| WPRIM
| ID: wpr-325569
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen the influenza A (H3N2) mimotopes by using phage display library.</p><p><b>METHODS</b>Using influenza A (H3N2) monoclonal antibody as selective molecule, a 7 mer phage peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.</p><p><b>RESULTS</b>21 positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was comfirmed as mimotope of influenza A (H3N2).</p><p><b>CONCLUSION</b>Influenza A (H3N2) mimotope was obtained by phage peptide library screening. The result provide a new approach for new Influenza virals vaccine development.</p>
Full text:
1
Database:
WPRIM
Main subject:
Chemistry
/
Epitope Mapping
/
Peptide Library
/
Allergy and Immunology
/
Influenza A Virus, H3N2 Subtype
/
Genetics
Type of study:
Diagnostic_studies
/
Screening_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2009
Document type:
Article