Polymorphism and multiplex amplification of 3 X-chromosome specific short tandem repeat loci / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 233-235, 2004.
Article
in Zh
| WPRIM
| ID: wpr-328911
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To devise a multiplex PCR system of three X-chromosome specific short tandem repeat (X-STR) loci and study the genetic polymorphism.</p><p><b>METHODS</b>DXS6799, DXS6804 and DXS6854 were amplified simultaneously using a multiplex system and were typed by polyacrylamide gel electrophoresis and silver staining.</p><p><b>RESULTS</b>A total of 262 male and 255 female individuals from Guangdong Han population were tested; each locus showed 7 alleles. 73 haplotypes were detected in the male individuals. The haplotype diversity reached 0.9674.</p><p><b>CONCLUSION</b>The 3 X-STR multiplex system is relatively abundant in polymorphic information for forensic identification and paternity testing.</p>
Full text:
1
Database:
WPRIM
Main subject:
Paternity
/
Polymorphism, Genetic
/
Haplotypes
/
Gene Amplification
/
Chromosome Mapping
/
Tandem Repeat Sequences
/
Chromosomes, Human, X
Limits:
Female
/
Humans
/
Male
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2004
Document type:
Article