Effect of demethylating treatment on cytotoxicity of NK-92MI cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 924-928, 2009.
Article
in Zh
| WPRIM
| ID: wpr-333995
Responsible library:
WPRO
ABSTRACT
In order to investigate the effect of demethylating treatment on the expression of inhibitory KIR and the cytolytic activity of NK-92MI cells, and to study the possible relationship between the demethylation of inhibitory kir gene and the function of NK cells. NK-92MI cells were treated with 5-azacytidine to induce DNA demethylation. The expression of KIR3DL1, KIR2DL2/KIR2DL3, KIR2DL1 and the viability of NK-92MI cells were detected by flow cytometry. The KIR3DL1 positive and the KIR3DL1 negative NK-92MI cells were also sorted by flow cytometry. The cytotoxicity of NK-92MI against K562 cells was detected by the LDH release assay. The results demonstrated that the expressions of KIR3DL1, KIR2DL2/KIR2DL3 and KIR2DL1 in NK-92MI cells all increased after treating with 1.0, 2.5 and 5 micromol/L of 5-azacytidine for 72 hours. And the cytotoxicity of NK-92MI against K562 cells decreased. In these dose range, 5-azacytidine did not influence the viability of NK-92MI cells. Additionally, the cytotoxicity of KIR3DL1 positive NK-92MI cells was lower than that of the KIR3DL1 negative cells. It is concluded that the demethylating treatment suppresses the cytotoxicity of NK-92MI cells through increasing the expression of inhibitory KIR.
Full text:
1
Database:
WPRIM
Main subject:
Killer Cells, Natural
/
Gene Expression Regulation
/
DNA Methylation
/
K562 Cells
/
Cytotoxicity, Immunologic
/
Allergy and Immunology
/
Receptors, KIR2DL1
/
Receptors, KIR2DL3
/
Receptors, KIR3DL1
/
Receptors, KIR3DL2
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2009
Document type:
Article