Effects of rhG-CSF mobilization on the polarization and migration of donor's CD4(+)T lymphocytes / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 470-474, 2014.
Article
in Zh
| WPRIM
| ID: wpr-349688
Responsible library:
WPRO
ABSTRACT
The polarization and migration of T lymphocytes involves the adhesive interaction of lymphocyte function-associated antigen 1(LFA-1) with its ligand intercellular adhesion molecule 1 (ICAM-1). This study was aimed to investigate the effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) mobilization on the polarization and migration of donor's CD4(+) T cells in peripheral blood. The peripheral blood mononuclear cells were collected from 10 healthy volunteers and 10 donors on the fifth day of mobilization with rhG-CSF. And the CD4(+)T cells were purified by miniMACS. The polarization and migration of CD4(+) T cells activated by stroma cell-derived factor -1α (SDF-1α) and ICAM-1 were detected by using inverted and confocal microscopes respectively. The results showed that the percentage of polarized CD4(+)T cells from donors(32.42 ± 4.91)% was lower than that from healthy controls(56.55 ± 5.35)% (P < 0.01), the migration velocity of CD4(+)T cells from donors (7.06 ± 1.44 µm/min) was also lower than that of healthy controls(9.05 ± 1.91 µm/min)(P < 0.01). It is concluded that the polarization and migration of CD4(+)T lymphocytes is impaired after rhG-CSF mobilization.
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
/
Tissue Donors
/
Recombinant Proteins
/
CD4-Positive T-Lymphocytes
/
Case-Control Studies
/
Cell Movement
/
Granulocyte Colony-Stimulating Factor
/
Cell Biology
Type of study:
Observational_studies
/
Risk_factors_studies
Limits:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2014
Document type:
Article