HPLC fingerprint chromatogram of leaves of Hawthorn / 中成药

ABSTRACT

AIM: To establish HPLC fingerprint chromatogram of leaves of Hawthorn and to evaluate the constituents and to provide the basis for identification. METHODS: Leaves of Hawthorn come from 10 production places were analysed to gain its contents,such as Vitexin,Hyperoside,2″-O-Rhamnosylvitexin,Rutin,4-O-Rhamnosylrutin,Chlorogenic acid,Quercetin 3-O-?-glucoside,4-O-Glucosylvitexiu,and internal reference selected Chlorogenic acid. RESULTS: The different leaves of Hawthorn showed different HPLC chromatogram. CONCLUSION: HPLC chromatogram can be used to distinguish the leaves of different species of Hawthorn and the content of the main characteristic components are various as production places vary.