Characterization of Echinostoma cinetorchis endoribonuclease, RNase H
The Korean Journal of Parasitology
; : 451-455, 2017.
Article
in En
| WPRIM
| ID: wpr-69358
Responsible library:
WPRO
ABSTRACT
Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other species. A recombinant EcRNH produced as a soluble form in Escherichia coli showed enzymatic activity to cleave the 3′-O-P bond of RNA in a DNA-RNA duplex, producing 3′-hydroxyl and 5′-phosphate. These findings may contribute to develop antisense oligonucleotides which could damage echinostomes and other flukes.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Parasites
/
Ribonucleases
/
Trematoda
/
RNA
/
Oligonucleotides, Antisense
/
DNA, Complementary
/
Ribonuclease H
/
Catalytic Domain
/
Echinostoma
/
Endoribonucleases
Language:
En
Journal:
The Korean Journal of Parasitology
Year:
2017
Document type:
Article