Effect of Coptidis Rhizoma on Endoplasmic Reticulum Stress Pathway PERK/ATF4/CHOP in Pancreas of Rats with Type 2 Diabetes Mellitus / 中国实验方剂学杂志

ABSTRACT

Objective: To observe the effect of Coptidis Rhizoma on blood sugar, inflammatory factors and protein kinase R-like endoplasmic reticulum kinase (PERK)/activating transcription factor 4(ATF4)/CCAAT/enhace-binding protein homologous protein (CHOP) pathway in type 2 diabetic mellitus rats. Method: The 80 SPF grade male rats were randomly divided into normal group, model group,metformin hydrochloride group, Coptidis Rhizoma group,with 20 rats in each group.The rats in the abnormal group were given "10-week high-sugar and high-fat diet+intraperitoneal injection of low dose streptozotocin (STZ)" regimen. Metformin hydrochloride group was given 0.2 g·kg-1·d-1, while rhizoma coptidis group was given 0.4 g·kg-1·d-1. Both model group and normal group were given the same amount of distilled water once a day.Fasting blood glucose (FBG),C reactive protein (CRP) and tumor necrosis factor-α (TNF-α) at an acute stage after 5-week intervention were determined by enzyme linked immunosorbent assay (ELISA) methods. Immunohistochemistry method was perforined with proteins glucoseregulated protein 78 (GRP78),ATF4,CHOP.Western blot assay was used to detect PERK,phosphorylated PKR like endoplasmic reticulum regulating kinase(p-PERK). Result: Compared with normal group,the FBG,CRP,TNF-α,p-PERK,GRP78,ATF4 and CHOP of model group improved(Pα,p-PERK,GRP78,ATF4,CHOP in contrast with model group(PConclusion: Coptidis Rhizoma can reduce blood sugar and reduce the levels of inflammatory factors,inhibit plasmic reticulum stress PERK/ATF4/CH0P pathway, and reduce CHOP, ATF4, GRP78, p-PERK expression in rats.