Buyang Huanwutang Alleviated Oxidative Stress Following Cerebral Ischemia/Reperfusion in Rats by Formyl Peptide Receptor 2 / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
; (24): 9-15, 2021.
Article
in Zh
| WPRIM
| ID: wpr-906013
Responsible library:
WPRO
ABSTRACT
Objective:
To investigate the role of formyl peptide receptor 2 (FPR2) in the inhibitory effects of Buyang Huanwutang (BYHWT) on the oxidative stress and its protective effects on cerebral ischemia-reperfusion in rats.Method:
Forty-eight male SD rats were randomly divided into sham group, model group, BYHWT group and BYHWT combined with FPR2 inhibitor (Boc-2) group. In the sham group, only the vessels were isolated. In other groups, the middle cerebral artery occlusion (MCAO) model was constructed using the modified Longa method and reperfused after 2 h of ischemia. BYHWT (16 g·kg<sup>-1</sup>) was given by gavaged twice daily after reperfusion in BYHWT group and BYHWT+Boc-2 group. Boc-2 (0.4 mg·kg<sup>-1</sup>) was injected intraperitoneally 30 min before surgery. Equal volume of saline were given instead in sham and model group. After 24 h of reperfusion, Fluoro-Jade C (FJC) staining was performed to observe the changes in the number of FJC-positive cells. Western blot was performed to detect the expression of apoptosis-related B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X (Bax), and cleaved aspartic acid cysteine proteolytic enzyme-3(Caspase-3). Besides, superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), and nitric oxide (NO) was measured. The mean fluorescence intensity of nicotinamide adenine dinucleotide phosphate Ⅱ(NADPH) oxidase 2 (NOX2) was examined by immunofluorescence.Result:
Compared with sham group, the model group showed increased number of FJC-positive cells (<italic>P</italic><0.01), decreased Bcl-2 expression (<italic>P</italic><0.01), increased Bax and cleaved Caspase-3 expression (<italic>P</italic><0.01), increased NO and MDA content (<italic>P</italic><0.05,<italic>P</italic><0.01), decreased GSH and SOD activities (<italic>P</italic><0.05,<italic>P</italic><0.01), and increased NOX2 expression (<italic>P</italic><0.01). Compared with model group, there were decreased FJC-positive cells (<italic>P</italic><0.01), up-regulated Bcl-2 expression (<italic>P</italic><0.01) with down-regulated cleaved Caspase-3 and Bax (<italic>P</italic><0.05,<italic>P</italic><0.01), decreased NO and MDA (<italic>P</italic><0.05,<italic>P</italic><0.01) with increased GSH and SOD (<italic>P</italic><0.01), and decreased NOX2 expression (<italic>P</italic><0.01) in the BYHWT group. All the above effects were partially blocked by Boc-2.Conclusion:
BYHWT can reduce oxidative stress injury and inhibit apoptosis in cerebral ischemia/reperfusion rats, which may be related with the down-regulation of NOX2 expression by FPR2.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Experimental Traditional Medical Formulae
Year:
2021
Document type:
Article