A preliminary study on estimation of postmortem interval according to beta-actin mRNA stability in rat / 法医学杂志
Journal of Forensic Medicine
; (6): 19-20, 2005.
Article
in Zh
| WPRIM
| ID: wpr-983064
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#In order to find a new parameter to estimate the postmortem interval, beta-actin mRNA in lung and thoracic muscle of rats was detected at different time point postmortem.@*METHODS@#Rats were killed by neck dislocation and left in a temperature controlling system at 21 degrees C for 12 days postmortem. Total RNA in lung and thoracic muscle at different time point was extracted and beta-actin mRNA expression was examined by RT-PCR. Semi-quantification analysis of the image of electrophoresis was performed to confirm the changes of beta-actin mRNA expression.@*RESULTS@#beta-actin mRNA in lung of rats still could be detected at 12 days postmortem, but-disappeared in thoracic muscle at 8 days postmortem.@*CONCLUSION@#The expression of beta-actin mRNA in lung and thoracic muscle could be a new parameter for estimation of PMI.
Full text:
1
Database:
WPRIM
Main subject:
Pectoralis Muscles
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Postmortem Changes
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Time Factors
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RNA, Messenger
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Actins
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Rats, Sprague-Dawley
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Reverse Transcriptase Polymerase Chain Reaction
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Forensic Medicine
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Lung
Limits:
Animals
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2005
Document type:
Article