Pin1 aggravates renal injury induced by ischemia and reperfusion in rats via Nrf2/HO-1 mediated endoplasmic reticulum stress
Acta cir. bras
; 37(1): e370101, 2022. ilus, graf
Article
in En
| LILACS, VETINDEX
| ID: biblio-1413330
Responsible library:
BR68.1
ABSTRACT
Purpose:
To investigate the role of peptidyl-prolyl cis/trans isomerase 1 (Pin1) on renal ischemia-reperfusion (I/R) injury and underlying mechanism.Methods:
By establishing the in vitro and in vivo models of renal I/R, the role of Pin1 was explored by using molecular assays.Results:
In renal I/R, endogenous Pin1 level was up-regulated in I/R-impaired kidney. Suppression of Pin1 with juglone afforded protection against I/R-mediated kidney dysfunction, and reduced I/R-induced endoplasmic reticulum (ER) stress in vivo. Consistent with the in vivo results, repression of Pin1 with juglone or gene knockdown with si-Pin1 conferred cytoprotection and restricted hypoxia/reoxygenation (H/R)-driven ER stress in HK-2 cells. Simultaneously, further study uncovered that Nrf-2/HO-1 signals was the association between Pin1 and ER stress in response to renal I/R. In addition, Nrf-2/HO-1 signal pathway was inactivated after kidney exposed to I/R, as indicated by the down-regulation of Nrf-2/HO-1 levels. Furthermore, inhibition of Pin1 remarkably rescued the inactivation ofNrf-2/HO-1.Conclusions:
Pin1 modulated I/R-mediated kidney injury in ER stress manner dependent on Nrf2-HO-1 pathway in I/R injury.Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
/
VETINDEX
Main subject:
Heme Oxygenase-1
/
NF-E2-Related Factor 2
/
NIMA-Interacting Peptidylprolyl Isomerase
/
Ischemia
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Acta cir. bras
Journal subject:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2022
Document type:
Article
Affiliation country:
China