The SGLT2 inhibitor empagliflozin attenuates atherosclerosis progression by inducing autophagy
J. physiol. biochem
; 80(1): 27-39, Feb. 2024. ilus, graf
Article
in En
| IBECS
| ID: ibc-EMG-563
Responsible library:
ES1.1
Localization: ES15.1 - BNCS
ABSTRACT
Cardiovascular disease due to atherosclerosis is one of the leading causes of death worldwide; however, the underlying mechanism has yet to be defined. The sodium-dependent glucose transporter 2 inhibitor (SGLT2i) empagliflozin is a new type of hypoglycemic drug. Recent studies have shown that empagliflozin not only reduces high glucose levels but also exerts cardiovascular-protective effects and slows the process of atherosclerosis. The purpose of this study was to elucidate the mechanism by which empagliflozin ameliorates atherosclerosis. Male apolipoprotein E-deficient (ApoE−/−) mice were fed a high-fat Western diet to establish an atherosclerosis model. The area and size of atherosclerotic lesions in ApoE−/− mice were then assessed by performing hematoxylineosin (HE) staining after empagliflozin treatment. Concurrently, oxidized low-density lipoprotein (oxLDL) was used to mimic atherosclerosis in three different types of cells. Then, following empagliflozin treatment of macrophage cells (RAW264.7), human aortic smooth muscle cells (HASMCs), and human umbilical vein endothelial cells (HUVECs), western blotting was applied to measure the levels of autophagy-related proteins and proinflammatory cytokines, and green fluorescent protein (GFP)-light chain 3 (LC3) puncta were detected using confocal microscopy to confirm autophagosome formation. Oil Red O staining was performed to detect the foaming of macrophages and HASMCs, and flow cytometry was used for the cell cycle analysis. 5-ethynyl-2′-deoxyuridine (EdU), cell counting kit-8 (CCK-8), and scratch assays were also performed to examine the proliferation and migration of HASMCs. Empagliflozin suppressed the progression of atherosclerotic lesions in ApoE−/− mice... (AU)
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1
Collection:
06-national
/
ES
Database:
IBECS
Main subject:
Autophagy
/
Atherosclerosis
/
Sodium-Glucose Transporter 2 Inhibitors
/
Research
/
Respiration, Artificial
/
Swimming
/
Carbon Dioxide
/
Nutrition Assessment
/
Coronavirus Infections
/
Blood Pressure Monitoring, Ambulatory
Type of study:
Etiology_studies
/
Observational_studies
/
Prevalence_studies
/
Prognostic_studies
/
Risk_factors_studies
Aspects:
Determinantes_sociais_saude
Limits:
Humans
/
Female
/
Male
/
Aged
/
Newborn
Country/Region as subject:
America do sul
/
Argentina
/
Asia
Language:
En
/
Es
Journal:
J. physiol. biochem
/
An. pediatr. (2003. Ed. impr.)
/
Arch. bronconeumol. (Ed. impr.)
/
Cir. Esp. (Ed. impr.)
/
Nutr. hosp
/
Rev. esp. patol. torac
Year:
2023
/
2022
/
2024
Document type:
Article