Characterization and partial purification of CD34+ progenitor cell ecto-phosphatidic acid phosphohydrolase.
Biochem Mol Biol Int
; 47(1): 9-23, 1999 Jan.
Article
in En
| MEDLINE
| ID: mdl-10092941
ABSTRACT
Phosphatidic acid and its hydrolysis product, diacylglycerol, play potentially vital roles as extracellular messengers in numerous cellular systems and may play a key role in regulating hematopoiesis. In this study, we describe an ecto-phosphatidic acid phosphohydrolase that potentially regulates cellular responses to phosphatidic acid on bone marrow derived human hematopoietic progenitors. We partially purified hematopoietic progenitor ecto-PAPase using a novel in-gel phosphatase assay and then characterized the enzyme on phenotypically defined subpopulations of hematopoietic CD34+ progenitors isolated by flow cytometry. The most pronounced PAPase activity was confined to uncommitted CD34+/CD38+ hematopoietic progenitors, which lacked the expression of other lineage-associated antigens. We conclude that hematopoietic progenitor cells at various stages of maturation possess a potent ecto-PAPase, an enzyme well positioned to regulate progenitor cell growth and differentiation induced by phosphatidic acid and related lipids.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphatidate Phosphatase
/
Hematopoietic Stem Cells
/
Antigens, CD34
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Biochem Mol Biol Int
Journal subject:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Year:
1999
Document type:
Article
Affiliation country:
Estados Unidos