Molecular epidemiological investigation of an outbreak of Campylobacter jejuni identifies a dominant clonal line within Scottish serotype HS55 populations.
Epidemiol Infect
; 122(3): 367-75, 1999 Jun.
Article
in En
| MEDLINE
| ID: mdl-10459638
ABSTRACT
Three molecular typing methods, pulsed-field gel electrophoresis (PFGE), ribotyping, and flagellin (flaA) gene typing, were used to discriminate within a group of 28 Campylobacter jejuni, heat-stable serotype 55 (HS55) isolates derived from cases of campylobacter enteritis occurring throughout Scotland, including 9 isolates associated with an outbreak. PFGE was found to be most discriminatory, identifying 6 distinct profiles, followed by ribotyping (5 profiles), and then flagellin gene typing (4 profiles). The coincidence of all three genotypic markers identified a dominant clonal line within the HS55 group, accounting for each of the outbreak strains, and for 9 of the 19 sporadic strains. A second, closely related, clonal line accounted for a further 5 of the sporadic strains, and also included the HS55 reference strain. Identification and monitoring of such clonal lines should facilitate more effective future epidemiological surveillance of C. jejuni.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Campylobacter Infections
/
Disease Outbreaks
/
Campylobacter jejuni
Limits:
Humans
Country/Region as subject:
Europa
Language:
En
Journal:
Epidemiol Infect
Journal subject:
DOENCAS TRANSMISSIVEIS
/
EPIDEMIOLOGIA
Year:
1999
Document type:
Article
Affiliation country:
Reino Unido