Validation of the fluorescence polarization assay as a serological test for the presumptive diagnosis of porcine brucellosis.
Vet Microbiol
; 68(3-4): 245-53, 1999 Aug 31.
Article
in En
| MEDLINE
| ID: mdl-10510043
ABSTRACT
Sera from Canadian pigs (brucellosis free, n = 14037) and sera from pigs infected with Brucella suis (n = 401) were tested by the buffered antigen plate agglutination test, the complement fixation test, an indirect and a competitive enzyme immunoassay and a fluorescence polarization assay. The results were analysed and assay sensitivity and specificity estimates were calculated. The sensitivity and specificity of the tests were as follows the buffered antigen plate agglutination test, 77.1 and 96.9%; the complement fixation test (considering anticomplementary sera as negative), 93.3 and 95.5%; the complement fixation test (considering anticomplementary sera as positive), 58.1 and 99.9%; the indirect enzyme immunoassay, 94.0 and 97.9%; the competitive enzyme immunoassay, 90.8 and 96.6%; and the fluorescence polarization assay, 93.5 and 97.2%; respectively. It was concluded that the fluorescence polarization assay was a valuable asset to the diagnosis of porcine brucellosis because of its accuracy, ease of performance and relative cost.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Swine Diseases
/
Brucella
/
Brucellosis
/
Fluorescence Polarization Immunoassay
/
Antibodies, Bacterial
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Vet Microbiol
Year:
1999
Document type:
Article