The yeast Rgd1p is a GTPase activating protein of the Rho3 and rho4 proteins.
FEBS Lett
; 459(3): 458-62, 1999 Oct 15.
Article
in En
| MEDLINE
| ID: mdl-10526184
ABSTRACT
The RGD1 gene, identified during sequencing of the Saccharomyces cerevisiae genome, encodes a protein with a Rho-GTPase activating protein (GAP) domain at the carboxy-terminal end. The Rgd1 protein showed two-hybrid interactions with the activated forms of Rho2p, Rho3p and Rho4p. Using in vitro assays, we demonstrated that Rgd1p stimulated the GTPase activity of both Rho3p and Rho4p; no stimulation was observed on Rho2p. In addition, the rho3Deltargd1Delta double mutant exhibited a dramatic growth defect compared to the single mutants, suggesting that Rgd1p has a GAP activity in vivo. The present study allowed the identification of the first GAP of Rho3p and Rho4p.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Saccharomyces cerevisiae
/
Fungal Proteins
/
GTP-Binding Proteins
/
Rho GTP-Binding Proteins
/
GTPase-Activating Proteins
/
Saccharomyces cerevisiae Proteins
Language:
En
Journal:
FEBS Lett
Year:
1999
Document type:
Article
Affiliation country:
Francia