Rab5 regulates motility of early endosomes on microtubules.
Nat Cell Biol
; 1(6): 376-82, 1999 Oct.
Article
in En
| MEDLINE
| ID: mdl-10559966
ABSTRACT
The small GTPase Rab5 regulates membrane docking and fusion in the early endocytic pathway. Here we reveal a new role for Rab5 in the regulation of endosome interactions with the microtubule network. Using Rab5 fused to green fluorescent protein we show that Rab5-positive endosomes move on microtubules in vivo. In vitro, Rab5 stimulates both association of early endosomes with microtubules and early-endosome motility towards the minus ends of microtubules. Moreover, similarly to endosome membrane docking and fusion, Rab5-dependent endosome movement depends on the phosphatidylinositol-3-OH kinase hVPS34. Thus, Rab5 functionally links regulation of membrane transport, motility and intracellular distribution of early endosomes.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Endosomes
/
Rab5 GTP-Binding Proteins
/
Endocytosis
/
Microtubules
Limits:
Humans
Language:
En
Journal:
Nat Cell Biol
Year:
1999
Document type:
Article
Affiliation country:
Alemania