Chromosomal localization of phospholipase A2 activating protein, an Ets2 target gene, to 9p21.
Genomics
; 62(3): 529-32, 1999 Dec 15.
Article
in En
| MEDLINE
| ID: mdl-10644453
ABSTRACT
A murine Ets2 target gene isolated by differential display cloning was identified as the phospholipase A2 activating protein (PLAA) gene. A 2.7-kb human cDNA demonstrating high homology to mouse and rat Plaa genes was then isolated and characterized. Human PLAA contains six WD-40 repeat motifs and three different protein kinase consensus domains. Fluorescence in situ hybridization (FISH) mapping placed PLAA on chromosome 9p21, a region frequently deleted in various cancers. A comprehensive mapping strategy was employed to define further the chromosomal localization of PLAA relative to CDKN2A within the 9p21 locus. Radiation hybrid mapping placed the gene 7.69 cR from WI-5735 (LOD >3.0), a marker in close proximity to CDKN2A and CDKN2B. Yeast artificial chromosome (YAC) mapping localized PLAA proximal to the CDKN2A/CDKN2B genes and to a region flanked by D9S171 and INFA commonly deleted in many neoplasms. Two YACs contained both PLAA and D9S259, a marker present in a second more proximal minimal deleted region observed in cutaneous melanoma and squamous cell lung carcinoma. Double-color fiber FISH mapping confirmed the location of PLAA centromeric to D9S171 and CDKN2A/CDKN2B. The mapping data suggest a possible tumor suppressor role for this gene.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Repressor Proteins
/
Transcription Factors
/
Chromosomes, Human, Pair 9
/
Proteins
/
Trans-Activators
/
Genes, Tumor Suppressor
/
Proto-Oncogene Proteins
/
Physical Chromosome Mapping
/
DNA-Binding Proteins
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Genomics
Journal subject:
GENETICA
Year:
1999
Document type:
Article
Affiliation country:
Canadá