Thoughts on the source of tissue on subsequent cell culture success.
Methods Cell Sci
; 22(1): 29-32, 2000 Mar.
Article
in En
| MEDLINE
| ID: mdl-10650332
ABSTRACT
This paper describes attempts to initiate equine adipocyte cultures from necropsy cases with varying intervals from time of death to isolation and culture. Equine adipocytes were isolated from 21 necropsy cases, regardless of the interval from time after death to establishment in primary ceiling cultures. However, while all cultures produced adipocytes, only 2 attempts to produce long-term equine adipocyte cultures from the subcutaneous rump fat depots were successful and not contaminated. Findings from these experiments indicate that it is possible to collect and culture equine adipocytes from necropsy cases with varying intervals of time of death to culturing provided that the issue of contamination is addressed. Viable cells were produced from tissue with an interval of 38.5 hours as well as 45 minutes. This result encourages the continuation of research using equine necropsy cases as a source of adipose tissue.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Specimen Handling
/
Adipose Tissue
/
Cell Culture Techniques
Limits:
Animals
Language:
En
Journal:
Methods Cell Sci
Journal subject:
BIOLOGIA MOLECULAR
Year:
2000
Document type:
Article
Affiliation country:
Estados Unidos