DNase I footprinting of the human interleukin-5 gene promoter.
Immunology
; 99(1): 101-8, 2000 Jan.
Article
in En
| MEDLINE
| ID: mdl-10651947
A characteristic feature of allergic asthma is the overexpression of the T helper type 2 (Th2) cytokines interleukin-4 (IL-4), IL-5 and IL-13 by T lymphocytes. Of these cytokines, IL-5 is critical for the growth, survival and recruitment of eosinophils which are thought to be responsible for the tissue damage observed in asthmatic airways. The expression of human IL-5 is primarily regulated at the transcriptional level; however, little is known about the mechanisms that control its transcription. Using nuclear extracts from allergen-specific human T-cell clones we have performed DNase I footprinting of the human IL-5 promoter in order to establish sites occupied by transcription factors. We show footprints covering the conserved lymphokine element 0 ¿(CLE0) -60 to -44 base pairs (bp) and GATA (-73 to -62 bp) elements, which have previously been identified to be important in the regulation of the murine IL-5 promoter. We also describe a footprint covering a considerably extended Octamer binding site (-249 to -217 bp), which encompasses two hitherto unidentified CCAAT/enhancer binding protein consensus binding sites. We have also identified a previously unknown Ets binding site (-274 to -264 bp). These novel data on the regions of the human IL-5 promoter that are bound by transcription factors should allow dissection of the regulatory mechanisms involved in the transcription of IL-5 in the T-helper lymphocytes of asthmatics.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Asthma
/
T-Lymphocytes
/
Gene Expression Regulation
/
Interleukin-5
/
Promoter Regions, Genetic
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Immunology
Year:
2000
Document type:
Article
Country of publication:
Reino Unido