Transcription factor PU.1 is necessary for development of thymic and myeloid progenitor-derived dendritic cells.
J Immunol
; 164(4): 1855-61, 2000 Feb 15.
Article
in En
| MEDLINE
| ID: mdl-10657634
Dendritic cells (DCs) are a heterogeneous population of cells that are specialized for Ag processing and presentation. These cells are believed to derive from both myeloid- and lymphoid-committed precursors. Normal human PBMC-derived, human CD14+ cell (monocyte)-derived, and mouse hematopoietic progenitor-derived DCs were shown to express the hematopoietic cell-restricted, ets family transcription factor PU.1. These populations represent myeloid progenitor-derived DCs. Hematopoietic progenitor cells from PU.1 gene-disrupted (null) mice were unable to generate MHC class IIhigh, CD11c+ myeloid-derived DCs in vitro. Mouse thymic DCs are proposed to be derived from a committed lymphoid progenitor cell that can give rise to T cells as well as DCs. Previously, we showed that CD4 and CD8 T cells developed in PU.1 null mice in a delayed manner and in reduced number. We examined the thymus of 10- to 12-day-old PU.1 null mice and found no evidence of DEC-205+, MIDC-8+ DCs in this tissue. Our findings indicate that PU.1 regulates the development of both thymic and myeloid progenitor-derived populations of DCs, and expand its known role in hematopoietic development.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thymus Gland
/
Dendritic Cells
/
Hematopoietic Stem Cells
/
Trans-Activators
/
Proto-Oncogene Proteins
Limits:
Animals
/
Humans
Language:
En
Journal:
J Immunol
Year:
2000
Document type:
Article
Affiliation country:
Estados Unidos
Country of publication:
Estados Unidos