AXIN1 mutations in hepatocellular carcinomas, and growth suppression in cancer cells by virus-mediated transfer of AXIN1.
Nat Genet
; 24(3): 245-50, 2000 Mar.
Article
in En
| MEDLINE
| ID: mdl-10700176
ABSTRACT
The Wnt signaling pathway is essential for development and organogenesis. Wnt signaling stabilizes beta-catenin, which accumulates in the cytoplasm, binds to 1-cell factor (TCF; also known as lymphocyte enhancer-binding factor, LEF) and then upregulates downstream genes. Mutations in CTNNB1 (encoding beta-catenin) or APC (adenomatous polyposis coli) have been reported in human neoplasms including colon cancers and hepatocellular carcinomas (HCCs). Because HCC5 tend to show accumulation of beta-catenin more often than mutations in CTNNB1, we looked for mutations in AXIN1, encoding a key factor for Wnt signaling, in 6 HCC cell lines and 100 primary HCC5. Among the 4 cell lines and 87 HCC5 in which we did not detect CTNNB1 mutations, we identified AXIN1 mutations in 3 cell lines and 6 mutations in 5 of the primary HCCs. In cell lines containing mutations in either gene, we observed increased DNA binding of TCF associated with beta-catenin in nuclei. Adenovirus mediated gene transfer of wild-type AXINI induced apoptosis in hepatocellular and colorectal cancer cells that had accumulated beta-catenin as a consequence of either APC, CTNNB1 or AXIN1 mutation, suggesting that axin may be an effective therapeutic molecule for suppressing growth of hepatocellular and colorectal cancers.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Repressor Proteins
/
DNA, Neoplasm
/
Signal Transduction
/
Proteins
/
Gene Expression Regulation, Neoplastic
/
Trans-Activators
/
Carcinoma, Hepatocellular
/
Zebrafish Proteins
/
Liver Neoplasms
/
Neoplasm Proteins
Type of study:
Prognostic_studies
Language:
En
Journal:
Nat Genet
Journal subject:
GENETICA MEDICA
Year:
2000
Document type:
Article
Affiliation country:
Japón