Generic polymerase chain reaction followed by DNA sequencing as a means of diagnosing bacteraemia.
Anaesth Intensive Care
; 28(1): 54-7, 2000 Feb.
Article
in En
| MEDLINE
| ID: mdl-10701038
ABSTRACT
There is increasing use of polymerase chain reaction techniques to diagnose infection. We report the use of polymerase chain reaction using a generic section of the bacterial 16S rDNA gene--followed by nucleotide sequencing--to determine the species of the infecting bacteria. In the first case, the clinical and microbiological diagnosis of meningococcal septicaemia was in agreement with the results from polymerase chain reaction technique. In the second case, a Yersinia enterocolitica bacteremia was detected by the polymerase chain reaction technique, but missed with conventional blood culture techniques.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymerase Chain Reaction
/
Bacteremia
Type of study:
Diagnostic_studies
Limits:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Anaesth Intensive Care
Year:
2000
Document type:
Article
Affiliation country:
Nueva Zelanda