Run-up of gamma-aminobutyric acid(C) responses in catfish retinal cone-horizontal cell axon-terminals is modulated by protein kinase A and C.
Neurosci Lett
; 282(1-2): 53-6, 2000 Mar 17.
Article
in En
| MEDLINE
| ID: mdl-10713394
ABSTRACT
Using whole-cell voltage-clamp techniques, we investigated the protein kinase modulation of gamma-aminobutyric acid(C) (GABA(C))-activated currents relating to run-up regulation in dissociated cone-horizontal cell (HC) axon-terminals from catfish retina. GABA induced an inward chloride current in cells voltage-clamped at -70 mV. With repetitive applications of 10 microM GABA, the peaks of the GABA responses increased up to approximately 135% of the control responses during a period of 10 min. Intracellular application of forskolin, an adenylate cyclase activator, decreased the run-up of GABA(C) responses. H8 dihydrochloride, a cAMP inhibitor, enhanced this run-up to 190% of the control responses. 1-oleoyl-2-acetyl-sn-glycerol, a protein kinase C activator, accelerated the run-up of GABA(C) responses. GF 109203X, a PKC inhibitor, decreased the run-up. These results suggest that retinal GABA(C) responses in cone-HC axon-terminals are modulated by both protein kinase A and C.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Kinase C
/
Receptors, GABA
/
Presynaptic Terminals
/
Cyclic AMP-Dependent Protein Kinases
/
Retinal Cone Photoreceptor Cells
Limits:
Animals
Language:
En
Journal:
Neurosci Lett
Year:
2000
Document type:
Article
Affiliation country:
Corea del Sur