DO11.10 and OT-II T cells recognize a C-terminal ovalbumin 323-339 epitope.
J Immunol
; 164(9): 4706-12, 2000 May 01.
Article
in En
| MEDLINE
| ID: mdl-10779776
ABSTRACT
The OVA323-339 epitope recognized by DO11.10 (H-2d) and OT-II (H-2b) T cells was investigated using amino- and carboxy-terminal truncations to locate the approximate ends of the epitopes and single amino acid substitutions of OVA323-339 to identify critical TCR contact residues of the OVA323-339 peptide. DO11.10 and OT-II T cells are both specific for a C-terminal epitope whose core encompasses amino acids 329-337. Amino acid 333 was identified as the primary TCR contact residue for both cells, and amino acid 331 was found to be an important secondary TCR contact residue; however, the importance of other secondary TCR contact residues and peptide flanking residues differ between the cells. Additional OVA323-339-specific clones were generated that recognized epitopes found in the N-terminal end or in the center of the peptide. These findings indicate that OVA323-339 can be presented by I-Ad in at least three binding registers. This study highlights some of the complexities of peptide Ags such as OVA323-339, which contain a nested set of overlapping T cell epitopes and MHC binding registers.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptide Fragments
/
T-Lymphocytes
/
Ovalbumin
/
Immunodominant Epitopes
/
Epitopes, T-Lymphocyte
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Immunol
Year:
2000
Document type:
Article
Affiliation country:
Estados Unidos