Monoclonal antibody 57B stains tumor tissues that express gene MAGE-A4.
Int J Cancer
; 86(6): 835-41, 2000 Jun 15.
Article
in En
| MEDLINE
| ID: mdl-10842198
ABSTRACT
Monoclonal antibody (Mab) 57B, which was raised against a recombinant MAGE-A3 protein, was tested for its ability to stain cells expressing various members of the MAGE-A gene family. COS-7 cells transfected with cDNAs encoding MAGE-A1, A2, A3, A4, A6, or A12 were stained, whereas those transfected with MAGE-A8, A9, A10, or A11 cDNAs were not. However, in tissue sections, we observed a different pattern of staining the antibody effectively stained the tumors that expressed MAGE-A4 and only these tumors, regardless of the expression of the other MAGE-A genes. It seems, therefore, that at the level of MAGE gene expression found in tumors, a level clearly lower than that observed in transfected COS cells, only the MAGE-A4 protein can be reliably detected. We conclude that the 57B Mab should be useful for tumor diagnosis related to therapeutic vaccination involving MAGE-A4.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Antibodies, Monoclonal
/
Antigens, Neoplasm
/
Neoplasms
Limits:
Animals
/
Humans
Language:
En
Journal:
Int J Cancer
Year:
2000
Document type:
Article
Affiliation country:
Bélgica